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 | Acesso ao texto completo restrito à biblioteca da Embrapa Soja. Para informações adicionais entre em contato com valeria.cardoso@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
10/02/2010 |
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Data da última atualização: |
03/08/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MENNA, P.; BARCELLOS, F. G.; HUNGRIA, M. |
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Afiliação: |
PÂMELA MENNA, CNPSo/UEL/CNPq-MCT; FERNANDO GOMES BARCELLOS, CNPSo/CNPq-MCT; MARIANGELA HUNGRIA DA CUNHA, CNPSo. |
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Título: |
Phylogeny and taxonomy of a diverse collection of Bradyrhizobium strains based on multilocus sequence analysis of the 16S rRNA gene, ITS region and glnll, recA, atpD and dnaK genes. |
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Ano de publicação: |
2009 |
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Fonte/Imprenta: |
International Journal of Systematic and Evolutionary Microbiology, Great Britain, v. 59, n. 12, p. 2934-2950, dez. 2009. |
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DOI: |
10.1099/ijs.0.009779-0 |
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Idioma: |
Inglês |
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Conteúdo: |
The genus Bradyrhizobium encompasses a variety of bacteria that can live in symbiotic and endophytic associations with legumes and non-legumes, and are characterized by physiological and symbiotic versatility and broad geographical distribution. However, despite indications of great genetic variability within the genus, only eight species have been described, mainly because of the highly conserved nature of the 16S rRNA gene. In this study, 169 strains isolated from 43 different legumes were analysed by rep-PCR with the BOX primer, by sequence analysis of the 16S rRNA gene and the 16S?23S rRNA intergenic transcribed spacer (ITS) and by multilocus sequence analysis (MLSA) of four housekeeping genes, glnII, recA, atpD and dnaK. Considering a cut-off at a level of 70 % similarity, 80 rep-PCR profiles were distinguished, which, together with type strains, were clustered at a very low level of similarity (24 %). In both single and concatenated analyses of the 16S rRNA gene and ITS sequences, two large groups were formed, with bootstrap support of 99 % in the concatenated analysis. The first group included the type and/or reference strains of Bradyrhizobium japonicum, B. betae, B. liaoningense, B. canariense and B. yuanmingense and B. japonicum USDA 110, and the second group included strains related to Bradyrhizobium elkanii USDA 76T, B. pachyrhizi PAC48T and B. jicamae PAC68T. Similar results were obtained with MLSA of glnII, recA, atpD and dnaK. Greatest variability was observed when the atpD gene was amplified, and five strains related to B. elkanii revealed a level of variability never reported before. Another important observation was that a group composed of strains USDA 110, SEMIA 5080 and SEMIA 6059, all isolated from soybean, clustered in all six trees with high bootstrap support and were quite distinct from the clusters that included B. japonicum USDA 6T. The results confirm that MLSA is a rapid and reliable way of providing information on phylogenetic relationships and of identifying rhizobial strains potentially representative of novel species. MenosThe genus Bradyrhizobium encompasses a variety of bacteria that can live in symbiotic and endophytic associations with legumes and non-legumes, and are characterized by physiological and symbiotic versatility and broad geographical distribution. However, despite indications of great genetic variability within the genus, only eight species have been described, mainly because of the highly conserved nature of the 16S rRNA gene. In this study, 169 strains isolated from 43 different legumes were analysed by rep-PCR with the BOX primer, by sequence analysis of the 16S rRNA gene and the 16S?23S rRNA intergenic transcribed spacer (ITS) and by multilocus sequence analysis (MLSA) of four housekeeping genes, glnII, recA, atpD and dnaK. Considering a cut-off at a level of 70 % similarity, 80 rep-PCR profiles were distinguished, which, together with type strains, were clustered at a very low level of similarity (24 %). In both single and concatenated analyses of the 16S rRNA gene and ITS sequences, two large groups were formed, with bootstrap support of 99 % in the concatenated analysis. The first group included the type and/or reference strains of Bradyrhizobium japonicum, B. betae, B. liaoningense, B. canariense and B. yuanmingense and B. japonicum USDA 110, and the second group included strains related to Bradyrhizobium elkanii USDA 76T, B. pachyrhizi PAC48T and B. jicamae PAC68T. Similar results were obtained with MLSA of glnII, recA, atpD and dnaK. Greatest variability was observed... Mostrar Tudo |
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Thesagro: |
Fixação de nitrogênio; Rhizobium. |
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Thesaurus Nal: |
Nitrogen fixation. |
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Categoria do assunto: |
V Taxonomia de Organismos |
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Marc: |
LEADER 02813naa a2200193 a 4500
001 1657500
005 2017-08-03
008 2009 bl uuuu u00u1 u #d
024 7 $a10.1099/ijs.0.009779-0$2DOI
100 1 $aMENNA, P.
245 $aPhylogeny and taxonomy of a diverse collection of Bradyrhizobium strains based on multilocus sequence analysis of the 16S rRNA gene, ITS region and glnll, recA, atpD and dnaK genes.
260 $c2009
520 $aThe genus Bradyrhizobium encompasses a variety of bacteria that can live in symbiotic and endophytic associations with legumes and non-legumes, and are characterized by physiological and symbiotic versatility and broad geographical distribution. However, despite indications of great genetic variability within the genus, only eight species have been described, mainly because of the highly conserved nature of the 16S rRNA gene. In this study, 169 strains isolated from 43 different legumes were analysed by rep-PCR with the BOX primer, by sequence analysis of the 16S rRNA gene and the 16S?23S rRNA intergenic transcribed spacer (ITS) and by multilocus sequence analysis (MLSA) of four housekeeping genes, glnII, recA, atpD and dnaK. Considering a cut-off at a level of 70 % similarity, 80 rep-PCR profiles were distinguished, which, together with type strains, were clustered at a very low level of similarity (24 %). In both single and concatenated analyses of the 16S rRNA gene and ITS sequences, two large groups were formed, with bootstrap support of 99 % in the concatenated analysis. The first group included the type and/or reference strains of Bradyrhizobium japonicum, B. betae, B. liaoningense, B. canariense and B. yuanmingense and B. japonicum USDA 110, and the second group included strains related to Bradyrhizobium elkanii USDA 76T, B. pachyrhizi PAC48T and B. jicamae PAC68T. Similar results were obtained with MLSA of glnII, recA, atpD and dnaK. Greatest variability was observed when the atpD gene was amplified, and five strains related to B. elkanii revealed a level of variability never reported before. Another important observation was that a group composed of strains USDA 110, SEMIA 5080 and SEMIA 6059, all isolated from soybean, clustered in all six trees with high bootstrap support and were quite distinct from the clusters that included B. japonicum USDA 6T. The results confirm that MLSA is a rapid and reliable way of providing information on phylogenetic relationships and of identifying rhizobial strains potentially representative of novel species.
650 $aNitrogen fixation
650 $aFixação de nitrogênio
650 $aRhizobium
700 1 $aBARCELLOS, F. G.
700 1 $aHUNGRIA, M.
773 $tInternational Journal of Systematic and Evolutionary Microbiology, Great Britain$gv. 59, n. 12, p. 2934-2950, dez. 2009.
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Registro original: |
Embrapa Soja (CNPSO) |
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Biblioteca(s): |
Embrapa Florestas. |
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Data corrente: |
10/11/2007 |
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Data da última atualização: |
18/03/2014 |
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Tipo da produção científica: |
Artigo em Anais de Congresso / Nota Técnica |
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Autoria: |
UHLMANN, A.; CURCIO, G. R.; POZZOBON, G. R.; BRAGHIROLLI, F.; STANO, F.; ATIQUE, M. S.; CEOLIN, L.; KNESS, A.; CAGLIONI, E.; QUINTANI, I. J.; VOLKMANN, A. |
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Afiliação: |
A. UHLMANN, FURB; GUSTAVO RIBAS CURCIO, CNPF; G. R. POZZOBON, FURB; F. BRAGHIROLLI, FURB; F. STANO, FURB; M. S. ATIQUE, FURB; L. CEOLIN, UFPR; A. KNESS, FURB; E. CAGLIONI, FURB; I. J. QUINTANI, FURB; A. VOLKMANN, FURB. |
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Título: |
Restauração de florestas fluviais na Bacia do Itajaí: avaliação do estabelecimento e desempenho inicial de nove espécies florestais em Cambissolo Háplico. |
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Ano de publicação: |
2007 |
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Fonte/Imprenta: |
In: CONGRESSO DE ECOLOGIA DO BRASIL, 8., 2007, Caxambu. Ecologia no tempo de mudanças globais: programa e anais. [S.l.]: Sociedade de Ecologia do Brasil, 2007. |
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Páginas: |
3 p. |
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Idioma: |
Português |
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Palavras-Chave: |
Avaliação; Bacia do Itajaí; Desempenho; Espécie florestal; Floresta fluvial; Restauração. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/99427/1/2007-AAC-RestauracaoCambissoloHaplico.pdf
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Marc: |
LEADER 01027nam a2200301 a 4500
001 1312328
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008 2007 bl uuuu u00u1 u #d
100 1 $aUHLMANN, A.
245 $aRestauração de florestas fluviais na Bacia do Itajaí$bavaliação do estabelecimento e desempenho inicial de nove espécies florestais em Cambissolo Háplico.$h[electronic resource]
260 $aIn: CONGRESSO DE ECOLOGIA DO BRASIL, 8., 2007, Caxambu. Ecologia no tempo de mudanças globais: programa e anais. [S.l.]: Sociedade de Ecologia do Brasil$c2007
300 $a3 p.
653 $aAvaliação
653 $aBacia do Itajaí
653 $aDesempenho
653 $aEspécie florestal
653 $aFloresta fluvial
653 $aRestauração
700 1 $aCURCIO, G. R.
700 1 $aPOZZOBON, G. R.
700 1 $aBRAGHIROLLI, F.
700 1 $aSTANO, F.
700 1 $aATIQUE, M. S.
700 1 $aCEOLIN, L.
700 1 $aKNESS, A.
700 1 $aCAGLIONI, E.
700 1 $aQUINTANI, I. J.
700 1 $aVOLKMANN, A.
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Embrapa Florestas (CNPF) |
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